Comparison of isotopic substitution methods for equilibrium and t-jump infrared studies of beta-hairpin peptide conformation.

Laser-induced temperature jump (T-jump) kinetics of relaxation was measured by absorbance of infrared (IR) detection for a set of peptide beta-hairpin, associated with hairpins Trpzip2, but it contains a label isotope single, (13) C to the amide C horizontal Lineo residue the selected both at the center and in the terminal strands of the hairpin. Variation in the behavior of a single labeled peptide compared with those previously reported for the double-la

beled variants. Although a single label does not produce an increase in spectral intensity, as seen to the labeling of cross-strand, IR frequency shift hairpin diagnostic still ongoing. If C horizontal Lineo at the beta-strands of the hairpin (between residues Trp) is labeled, the dynamic behavior of the local fashion similar to the results obtained with the double label in terms of the relaxation time and energy and closely track the kinetics of activation of beta-strand component.

This means that both the property, local secondary structure (change varphi, psi), or cross-strand clutch is activated by the formation of H-bonded strands and loosening with the same kinetic mechanism. Single residue at position terminal label have different behaviors and less able to detect because it overlaps with the component (12) C, in contrast with the double label involving the position, which is enhanced due to coupling. DFT-based spectral simulation using NMR structure of Trpzip2C showed that single-labeled peptides must have a roughly equivalent (12) C band but (13) C mode frequency will vary with the position of the sequence. effective solvent correction using COSMO yield significant changes in frequency but not in relative isotope shift in the spectrum obtained we calculated. The sequence dependency label position is shown to be more discriminatory than the variation thermodynamics kinetics change.

DL-Dithiothreitol/DTT
ADC-P-081 unit Ask for price

DL-Dithiothreitol, 99%
GC1199-100G 100 g
EUR 361.2

DL-Dithiothreitol, 99%
GC1199-10G 10 g
EUR 93.6

DL-Dithiothreitol, 99%
GC1199-25G 25 g
EUR 141.6

DL-Dithiothreitol, 99%
GC1199-50G 50 g
EUR 217.2

DL-Dithiothreitol, 99%
GC1199-5G 5 g
EUR 69.6

DL-Dithiothreitol, 98%
GC3928-5G 5 g
EUR 64.8

Dithiothreitol 1 g
17131801 EACH
EUR 55.86

Dithiothreitol 5 g
17131802 EACH
EUR 157.32

1G Dithiothreitol (DTT)
NAT1066 1G
EUR 48.79

5G Dithiothreitol (DTT)
NAT1068 5G
EUR 140.22

DTT, 1,4-Dithiothreitol, Diaxone free
D0505-001 1g
EUR 111.6

DTT, 1,4-Dithiothreitol, Diaxone free
D0505-005 5g
EUR 148.8

DTT, 1,4-Dithiothreitol, Diaxone free
D0505-010 10g
EUR 217.2

DTT, 1,4-Dithiothreitol, Diaxone free
D0505-050 50g
EUR 604.8

DTT, 1,4-Dithiothreitol, Diaxone free
D0505-100 100g
EUR 842.4

DL-Dithiothreitol/DTT - biotech grade
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DL-Dithiothreitol/DTT - pure grade
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